ASCC Research Portfolio

Purpose

Core Services provides researchers at the ASCC with specialised equipment, instrumentation and laboratory support that would otherwise be too costly, complex, or inaccessible to individual laboratories. These services include Animal Services Facility, Central Services, Flow Cytometry and a Human Embryonic Stem Cell Facility. The Cores rely on feedback and direction from the research groups and are committed to enhancing the research performed by all ASCC scientists.

Description

The Animal Services Facility provides support to research groups engaged in animal experimentation. Services provided include assisting with the organisation of ethics applications and amendments, colony maintenance, procurement of animals for research, oversight of animal holding facilities, provision of Standard Operating Procedures (SOPs), training and a wide variety of technical procedures and advice.

Central Services undertakes the cleaning and sterilisation of reusable labware and provides a range of laboratory consumables for researchers. Services include custom sterilisation and provision of separate tissue culture and non tissue culture laboratory glass and plastic ware. They have a range of in house products from PBS buffers to basal media for mammalian and bacterial culture. Central Services provides convenient, reliable, and quality service that is also cost effective for the end user.

The ASCC's Flow Cytometry facility enhances scientific research by providing high quality flow cytometry instrumentation and expertise to the stem cell research community. Flow cytometry is a laser based technology for analysing and sorting cells. The flow cytometry facility currently has two high speed cell sorters and one dual laser analyser, see attached PDF for full description of the facilities capabilities.

Flow Cytometry, PDF

Direct access to the Flow Cytometry Booking System

The main function of the Human Embryonic Stem Cell (hESC) Facility is the characterisation and provision of hES cells to be used by ASCC researchers. The Facility is also committed to the provision of excellent technical advice and training to ASCC researchers and collaborators to enhance their research efforts in the field of hESCs. With operations at Monash University and the University of Queensland, the Facility provides the following services:

  • weekly hESC production in manual, enzymatic and feeder free culture conditions
  • quality control - qPCR, immunocytochemistry, karyotyping and preparation of hESC's for teratoma formation
  • derivation and propagation of primary mouse embryonic fibroblast (mEF) feeder cell lines
  • cell banking incorporating freezing of hESC and mEF for distribution, thawing and culture of frozen cell lines
  • culture of researchers individual manipulated/modified hESC lines

The hESC Facility also provides a comprehensive 4-day training workshop with hands-on, laboratory-based training in the propagation and manipulation of hESC lines and MEF feeders which support hESC growth in culture. Further information on training is available on our website.

 

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