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Stem Cell Expansion Laboratory
Group Leader
David Haylock, PhD
Senior Scientist
Contact Details
Email: david.haylock@stemcellcentre.edu.au
To find out more about David Haylock, PhD, click here.
Lab Members
Jessica Andrade– CRC Polymers Research Assistant
Rebecca Neaves – PhD Student
Research Focus
The objective of our research is development of innovative cell therapies based on haemopoietic stem cells.
Overview
The overall focus of the laboratory is to dissect the intrinsic and extrinsic aspects of haemopoietic regulation that are attributable to haemopoietic stem cell (HSC) and the HSC niche. Specifically, this involves defining molecules comprising the HSC niche, as well as the mechanisms involved in attracting, retaining, regulating and releasing HSC from the niche.
An understanding of these mechanisms will allow us to develop strategies for constructing an artificial niche recapitulating the interactions evident in situ.
This will provide opportunities to gain a further insight into the mechanisms influencing HSC within the niche, as well as the potential to manipulate HSC ex vivo for therapeutic purposes. A longer term goal is to encompass the collaborative development of bioreactors for large scale HSC manipulation. A comprehensive understanding of the HSC niche as well as the mechanisms involved in HSC regulation will allow us to more critically analyse aberrant haemopoiesis, and specifically leukaemia. Furthermore, an understanding of the HSC niche will allow us to collaboratively analyse other stem cell niches, both normal and neoplastic.
Funding
Leukemia and Lymphoma Society of America
Cass Foundation
Selected Publications
Haylock DN, B Williams, HM Johnston, MCP Liu, KE Rutherford, GA Whitty, PJ Simmons, I Bertoncello, SK Nilsson. HSC with higher hemopoietic potential reside at the bone marrow endosteum. Stem Cells 2007; 25:1062-1069.
Haylock DN, Nilsson SK. The role of hyaluronic acid in hemopoietic stem cell biology. Reg Med 2006; 1(4): 437-445.
Haylock DN, Nilsson SK. Osteopontin: a bridge between bone and blood. Br J Haematol. 2006; 134(5): 467-74.
Boyle AJ, Whitbourn R, Schlicht S, Krum H, Kocher A, Nandurkar H, et al. Intra-coronary high-dose CD34+ stem cells in patients with chronic ischemic heart disease: a 12-month follow-up. Int J Cardiol. 2006; 109(1):21-7.
Haylock DN, Nilsson SK. Stem cell regulation by the hematopoietic stem cell niche. Cell Cycle. 2005; 4(10): 1353-5.
Nilsson SK, Johnston HM, Whitty GA, Williams B, Denhardt DT, Bertoncello I, et al. Osteopontin, a Key Component of the Hemopoietic Stem Cell Niche and Negative Regulator of Primitive Hemopoietic Progenitor Cells. Blood. 2005; 106(4):1232-9.
Haylock DN, Simmons PJ. Approaches to Hematopoietic Stem Cell Separation and Expansion. In: Lanza R, Blau H, Melton D, Moore M, Thomas ED, Verfaillie C, Weissman I, West M, editors. Handbook of Stem Cells, Vol 2, Adult and Fetal. Elsevier Academic Press; 2004. p 615-631.
Prince HM, Simmons PJ, Whitty G, Wall DP, Barber L, Toner GC, et al. Improved hemopoietic recovery following transplantation with ex vivo expanded mobilized blood cells. Br J Haematol. 2004; 126:536-45.
Nilsson SK, Haylock DN, Johnston HM, Occiodoro T, Brown TJ, Simmons PJ. Hyaluron is synthesised by primitive hemopoietic cells, participates in their lodgment at the endosteum following transplantation, and is involved in the regulation of their proliferation and differentiation in vitro. Blood. 2003; 101:856-862.
Prince HM, Wall D, Rishin D, Toner GC, Seymour JF, Blakey D, et al. CliniMACS CD34-selected cells to support multiple cycles of high-dose therapy. Cytotherapy. 2002; 4:147-155.
Prince HM, Bashford J, Wall D, Rishin D, Parker N, Toner GC, et al. Isolex 300i CD34-selected cells to support multiple cycles of high-dose therapy. Cytotherapy. 2002; 4:137-145.
Lévesque JP, Takamatsu Y, Nilsson SK, Haylock DN, Simmons PJ. Vascular cell adhesion molecule-1 (CD106) is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor. Blood 2001; 98:1289-97.
Simmons PJ, Lévesque JP, Haylock DN. Mucin-like molecules as modulators of the survival and proliferation of primitive hematopoietic cells. Ann NY Acad Sci 2001; 938:196-206; discussion 206-7.
Ramshaw HS, Haylock DN, Swart B, Gronthos S, Horsfall MJ, Nuitta S, et al. Monoclonal antibody BB9 raised against bone marrow stromal cells identifies a cell-surface glycoprotein expressed by primitive human hemopoietic progenitors. Exp Hematol 2001; 29:981-992.
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